Transmitted Light Microscopy Optical Pathways

This tutorial explores the optical pathways in a transmitted light microscope. Instructions and a discussion about how to operate this tutorial appear immediately below the window.

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To operate the tutorial, use the slider bars beneath the microscope to control the aperture of the field diaphragm (left slider), the aperture (condenser) diaphragm (middle slider), and the illumination intensity (right slider). The button controls whether the internal ("inside") or external ("outside") view of the microscope is selected.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Matthew J. Parry-Hill, John C. Long, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.