Fluorescence with Multiple Fluorochromes

This interactive tutorial explores the effects of fluorescence cube filtration of excitation/emission spectra on specimens stained with multiple fluorochromes.

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To operate the tutorial, first use the pull-down menu to choose a sample. Next, use your mouse cursor to select a fluorescence filter cube using the radio buttons. The image will change in the microscope port to report how the sample appears in the microscope when illuminated with the chosen filter cube.

Contributing Authors

Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.

Kirill I. Tchourioukanov and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.